Real-time visualization of structural dynamics of synapses in live cells in vivo
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Real-time visualization of structural dynamics of synapses in live cells in vivo

Prof. Won Do Heo’s group at KAIST has developed ‘SynapShot,’ a method for visualizing the structural dynamics of intact synapses by combining dimerization-dependent FPs (ddFPs) with engineered synaptic adhesion molecules. SynapShot allows real-time monitoring of reversible and bidirectional changes of synaptic contacts under physiological stimulation. The application of green and red ddFPs in SynapShot enables simultaneous visualization of two distinct populations of synapses. Notably, the red-shifted SynapShot is highly compatible with blue light-based optogenetic techniques, allowing for vis...read more